1. Download the imageJ, https://imagej.net/Fiji/Downloads

2. Unzip the

3. Installing a plug-in
(1) https://imagej.nih.gov/ij/plugins/index.html download plug-in you need. Open Toolsets or tools.

![在这里插入图片描述](https://img-blog.csdnimg.cn/20201013153831165.png?x-oss-process=image/watermark,type_ZmFuZ3poZW5naGVpdGk,shadow_10,text_aHR0cHM6Ly9ibG9nLmNzZG4ubmV0L3dlaXhpbl80MTI0MjEyOA==,size_16,color_FFFFFF,t_70#pic_center)

(2) click to open the required plug-in: copy content;

(3) create a.txt file under macros folder;

open image j, click on the top plugins-new-macro,
in the macro interface open. TXT file
copy the TXT content before opening to the open. TXT blank interface

（4）ImageJ菜单栏单击 Plugins-Macros-Install；选择刚刚创建的.txt文件，打开，安装完毕！

original text file

$ cat test              
jason
jason
jason
fffff
jason

Method 1: sort -u

after removal of repetition

sort -u test
fffff
jason

notice that the order is disrupted

sort test|uniq

after removal of repetition

$sort test |uniq 
fffff
jason

note that the order is disrupted, the principle and method are the same

method three: awk ‘! A [$0] + + ‘

after removal of repetition

$ awk '!a[$0]++' test
jason
fffff

order remains the same, file deduplication example

awk '!a[$0]++' test.txt >test.txt.tmp && mv -f test.txt.tmp test.txt

where awk USES a temporary file to overwrite the result

specific principle is as follows:

awk’s program instructions consist of patterns and actions, in the form of Pattern {Action}. If the Action is omitted, print $0 will be executed by default.

Pattern:

can be used here to remove repetition

!a[$0]++

In awk, for uninitialized array variables, an initial value of 0 is assigned to them during numerical operations, so a[$0]=0, and the ++ operator is characterized by first value and then 1, so Pattern is equivalent to

!0

and 0 is false,! In order to get the reverse, the final result of the whole Pattern is 1, which is equivalent to if(1). The Pattern matching is successful, and the current record is output. For the DUP file, the processing method of the first three records is the same.

when the data “Jason” in line 2 is read, a[$0]=1, and the result after taking the reverse is 0, that is, the Pattern is 0, and the Pattern matching fails, so this record is not output, and the subsequent data is followed by the same, and the duplicate lines in the file are finally removed successfully.

is recorded as follows:

1. Download the appropriate version of GCC from the official website, http://gcc.gnu.org.

2. Unpack the

# sudo tar ZXFV GCC – 4.4.6. Tar. Gz

3. Go to the unzip directory, mine is ~/local/gcc-4.4.6, and I have an executable called configure, with the following configuration:

#./configure — prefix =/usr/bin/GCC – 4.4.6 – enable – language = c, c + +, Java

(where /usr/bin/gcc-4.4.6 is the directory to install GCC, followed by –enable-language=c,c++, Java is set to install compilable language)

Notice that the configure success sign shows that the makefile was successfully generated and that you’ll have an additional makefile in your directory so you can proceed to the next

---

error: said GMP and MPFR

cannot be found

to download and install dependencies:
ftp://gcc.gnu.org/pub/gcc/infrastructure

GMP – 4.3.2. Tar..bz2

MPFR – 2.4.2. Tar..bz2

unzip installation dependency:

tar -jxvf gmp-4.3.2.tar.bz2

mkdir /usr/local/gmp-4.3.2

cd ./gmp-4.3.2

./configure --prefix=/usr/local/gmp-4.3.2

make

make install



tar -jxvf mpfr-2.4.2.tar.bz2

mkdir /usr/local/mpfr-2.4.2

cd ./mpfr-2.4.2

./configure --prefix=/usr/local/mpfr-2.4.2 --with-gmp=/usr/local/gmp-4.3.2

make

make install

error: m4

cannot be found
Install GMP under ubuntu

. Configure :error:no usable m4 in$path or /user/5bin solution

is easy, because you don’t have m4, just install it.

sudo apt-get install m4

4.#make

this is the longest step, so long that I couldn’t wait to go back to sleep last night…

note: error may also occur in make. Generally, it is also necessary to configure the environment or missing files. It is OK to install whatever is needed directly

5. # make install

6. After the end, establish the connection of gcc4.4.6 version, command:

# ln -s/usr/bin/GCC – 4.4.6/bin/GCC/usr/bin/GCC
Check out the current version of GCC:

#gcc –version